Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody is a Rabbit antibody targeting Phospho-YAP1 (Ser127). Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Horse,Rabbit)

1. Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-YAP1 (Ser127) Polyclonal Antibody, Unconjugated (TMAB-01530) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human YAP around the phosphorylation site of Ser127

Human

Signal transduction,Proto-oncogenes,Transcription Factors,Cytoplasmic,Src Family