Anti-Phospho-VEGFR2/KDR (Tyr1175) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1175). Anti-Phospho-VEGFR2/KDR (Tyr1175) Polyclonal Antibody can be used in WB,FCM.

IgG

Mouse,Rat,Human (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Sample:
Lane 1: Placenta (Mouse) Lysate at 40 μg
Lane 2: Adrenal gland (Rat) Lysate at 40 μg
Primary: Anti-phospho-VEGFR2 (Tyr1175) (TMAB-01529) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 220 kDa
2. Blank control: Huvec.
Primary Antibody (green line): Rabbit Anti-phospho-VEGFR2 (Tyr1175) antibody (TMAB-01529)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2ug/test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human VEGFR2 around the phosphorylation site of Tyr1175

Human

Response to hypoxia,VEGF,Angiogenic growth factors,Growth factor receptors,Endothelial Cell Markers,VEGF Receptors,Endothelial Cells,Hypoxia,Host Virus Interaction,VEGF,Receptor Tyrosine Kinases,Endothelial Markers,Surface Molecules