Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody is a Rabbit antibody targeting Phospho-VEGFR2/KDR (Tyr1059). Anti-Phospho-VEGFR2/KDR (Tyr1059) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse,Human (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-VEGFR Polyclonal Antibody, Unconjugated (TMAB-01528) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Huvec.
Primary Antibody (green line): Rabbit Anti-phospho-VEGF receptor 2 (Tyr1059) antibody (TMAB-01528)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
HUVEC (Hu) lysate at 30 μg
Primary: Anti-phospho-VEGF receptor 2 (Tyr1059) (TMAB-01528) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 147 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human VEGF receptor 2 around the phosphorylation site of Tyr1059

Human

Response to hypoxia,VEGF,Angiogenic growth factors,Growth factor receptors,Endothelial Cell Markers,VEGF Receptors,Endothelial Cells,Hypoxia,Host Virus Interaction,VEGF,Receptor Tyrosine Kinases,Endothelial Markers,Surface Molecules