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Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587)
还可以产品编号 TMAH-00913
别名 Phospho-JAK2 (Y1007, 1008)
Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) 是一种抗体，靶向 Phospho-JAK2 (Tyr1007, 1008)。Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) 可用于 ELISA, WB, IHC, IP。
Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587)
Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587)
还可以产品编号 TMAH-00913 别名 Phospho-JAK2 (Y1007, 1008)
Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) 是一种抗体，靶向 Phospho-JAK2 (Tyr1007, 1008)。Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) 可用于 ELISA, WB, IHC, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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抗原信息
| 产品描述 | Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) is an antibody targeting Phospho-JAK2 (Tyr1007, 1008). Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (5T587) can be used in ELISA, WB, IHC, IP. |
| 别名 | Phospho-JAK2 (Y1007, 1008) |
| Ig Type | Rabbit IgG |
| 克隆号 | 5T587 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in Hela whole cell lysate,A549 whole cell lysate(treated with Pervanadate or not) -All lanes Phospho-JAK2 antibody at 0.75μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 120 KDa -Observed band size: 120 KDa 2. IHC image of TMAH-00913 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00913 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunoprecipitating Phospho-JAK2 in Hela whole cell lysate treated with Pervanadate -Lane 1: Rabbit control IgG(1μg)instead of TMAH-00913 in Hela whole cell lysate treated with Pervanadate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00913(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg) -Lane 3: Hela whole cell lysate treated with Pervanadate(20μg) |
| 应用 | ELISA, WB, IHC, IP |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Endomembrane system; Peripheral membrane protein. Cytoplasm. Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-JAK2 (Y1007 + Y1008) |
| 抗原种属 | Human |
| 基因ID | 3717 |
| Uniprot ID | |
| 研究领域 | Cell Biology |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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