Anti-PARK7/DJ-1 Polyclonal Antibody is a Rabbit antibody targeting PARK7/DJ-1. Anti-PARK7/DJ-1 Polyclonal Antibody can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse)

1. Blank control: 293T cells (blue). Primary Antibody: Rabbit Anti-PARK7/CAP1 antibody (TMAB-01332), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange),used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (TMAB-01332, 0.2 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (TMAB-01332) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-CAP1/PARK7 Polyclonal Antibody, Unconjugated (TMAB-01332) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. The blue histogram is unstained cells (Hepg2 cells)
concentration 1:50
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibody
plus secondary antibody.
The green histogram is cells stained with Rabbit Anti-PARK7/CAP1 antibody (TMAB-01332) plus secondary antibody.
5. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Thyroid gland Rat) Lysate at 40 μg
Lane 5: Kidney (Rat) Lysate at 40 μg
Lane 6: Liver (Rat) Lysate at 40 μg
Lane 7: Hela (Human) Cell Lysate at 30 μg
Lane 8: U937 (Human) Cell Lysate at 30 μg
Lane 9: K562 (Human) Cell Lysate at 30 μg
Lane 10: HL60 (Human) Cell Lysate at 30 μg
Primary:
Anti-PARK7/DJ1 (TMAB-01332) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
6. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (TMAB-01332) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PARK7) Polyclonal Antibody, Unconjugated (TMAB-01332) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CAP1

Human

PARK7

Parkinson disease protein 7

Small G proteins,DNA / RNA binding,Oxidative stress,Parkin / PARK,Regulators

Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.