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Anti-GPI Antibody (6K139) 是一种 Rabbit 抗体,靶向 GPI。Anti-GPI Antibody (6K139) 可用于 WB,ICC,IF,IHC,FCM。
Anti-GPI Antibody (6K139) 是一种 Rabbit 抗体,靶向 GPI。Anti-GPI Antibody (6K139) 可用于 WB,ICC,IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-GPI Antibody (6K139) is a Rabbit antibody targeting GPI. Anti-GPI Antibody (6K139) can be used in WB,ICC,IF,IHC,FCM. |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin. 5. ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein: human Glucose 6 phosphate isomerase 300-500aa |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 63 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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