Anti-FGF-2 Polyclonal Antibody is a Rabbit antibody targeting FGF-2. Anti-FGF-2 Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse (predicted:Rat,Chicken,Cow,Rabbit,Sheep)

1. Sample:
HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-bFGF (TMAB-00669) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
2. Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-bFGF antibody (TMAB-00669)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human bFGF

Human

FGF2

Fibroblast growth factor 2

FGF,Angiogenic growth factors,FGF,Kidney development,Neurogenesis,FGF,Extracellular

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.