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Anti-DDX5 Antibody (7B467)
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产品编号 TMAC-01133
Anti-DDX5 Antibody (7B467) 是一种 Rabbit 抗体，靶向 DDX5。Anti-DDX5 Antibody (7B467) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-DDX5 Antibody (7B467)
Anti-DDX5 Antibody (7B467)
还可以产品编号 TMAC-01133
Anti-DDX5 Antibody (7B467) 是一种 Rabbit 抗体，靶向 DDX5。Anti-DDX5 Antibody (7B467) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
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| 产品描述 | Anti-DDX5 Antibody (7B467) is a Rabbit antibody targeting DDX5. Anti-DDX5 Antibody (7B467) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1,000 dilution. Positive control: Lane1: PC-12, Lane2: MEF, Lane3: SH-SY5Y, Lane4: NIH-3T3, Lane5: Mouse brain. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DDX5 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin. 5. ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining DDX5 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining DDX5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of SH-SY5Y cells with DDX5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | p68 RNA helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA helicase activity. p68 RNA helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA helicase increases in serum-induced quiescent cell lines. p68 RNA helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA helicase associates with transcriptional coactivators CBP and p300. p68 RNA helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA helicase and fibrillarin colocalize to nascent nucleoli. p68 RNA helicase may function as a heterodimer with p72 RNA helicase. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 69/60 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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