Anti-CD73 Polyclonal Antibody is a Rabbit antibody targeting CD73. Anti-CD73 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)

1. Sample:
Lane 1: A431 (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-CD73 (TMAB-00386) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63/71 kDa
Observed band size: 65 kDa
2. Sample: Kidney (mouse) l Lysate at 40 μg
Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 65 kDa
3. Sample: HepG2 (human) cell Lysate at 40 μg
Primary: Anti-CD73 (TMAB-00386) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
4. Blank control (blue line): Raji (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (TMAB-00386)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD73/NT5

Human

Associated Proteins,Leukocyte recruitment