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Anti-Beta-Catenin Antibody (8B317)
还可以产品编号 TMAY-01820
Anti-Beta-Catenin Antibody (8B317) 是一种 Rabbit 抗体，靶向 Beta-Catenin。Anti-Beta-Catenin Antibody (8B317) 可用于 ELISA,IHC-P,FCM,ICC/IF。
Anti-Beta-Catenin Antibody (8B317)
Anti-Beta-Catenin Antibody (8B317)
还可以产品编号 TMAY-01820
Anti-Beta-Catenin Antibody (8B317) 是一种 Rabbit 抗体，靶向 Beta-Catenin。Anti-Beta-Catenin Antibody (8B317) 可用于 ELISA,IHC-P,FCM,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Beta-Catenin Antibody (8B317) is a Rabbit antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (8B317) can be used in ELISA,IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8B317 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CTNNB1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CTNNB1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. 2. Immunochemical staining of human CTNNB1 in human skin with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of human CTNNB1 in human colon with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 4. Flow cytometric analysis of Human CTNNB1 (β-Catenin) expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CTNNB1 (β-Catenin), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA,IHC-P,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human beta-Catenin / CTNNB1 (rh beta-Catenin / CTNNB1; TMPY-02205; P35222-1; Met1-Leu781). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human beta-Catenin / CTNNB1 protein (TMPY-02205) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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