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Anti-Argonaute-2 Antibody (1L369)

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Anti-Argonaute-2 Antibody (1L369)
产品编号 TMAC-00265
别名 Q10, EIF2C2, argonaute RISC catalytic component 2, Argonaute 2

Anti-Argonaute-2 Antibody (1L369) 是一种 Rabbit 抗体,靶向 Argonaute-2。Anti-Argonaute-2 Antibody (1L369) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-Argonaute-2 Antibody (1L369)

Anti-Argonaute-2 Antibody (1L369)

Rating icon 还可以
Anti-Argonaute-2 Antibody (1L369)
产品编号 TMAC-00265 别名 Q10, EIF2C2, argonaute RISC catalytic component 2, Argonaute 2

Anti-Argonaute-2 Antibody (1L369) 是一种 Rabbit 抗体,靶向 Argonaute-2。Anti-Argonaute-2 Antibody (1L369) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Argonaute-2 Antibody (1L369) is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Antibody (1L369) can be used in WB,ICC/IF,IHC,IP,FCM.
别名Q10, EIF2C2, argonaute RISC catalytic component 2, Argonaute 2
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7.
2. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
5. ICC staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 97 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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