Anti-Arginase 1/ARG1 Antibody (5R257) is a Rabbit antibody targeting Arginase 1/ARG1. Anti-Arginase 1/ARG1 Antibody (5R257) can be used in WB,ELISA,IHC-P,FCM,ICC/IF.

Monoclonal Rabbit IgG

5R257

Human

1. Immunochemical staining of human ARG1 in human liver with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

2. Immunochemical staining of human ARG1 in human kidney with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

3. Anti-ARG1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:35 kDa.

-Observed band size:40 kDa.

4. Flow cytometric analysis of Human ARG1 expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ARG1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

5. Immunofluorescence staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ARG1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).

WB,ELISA,IHC-P,FCM,ICC/IF

WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IHC-P: 1:500-1:2500; ICC-IF: 1:20-1:100; FCM: 1:100-1:500

Monoclonal

Unconjugated

Recombinant Human ARG1 / Arginase 1 protein (TMPY-02168)

Human