购物车
- 全部删除
您的购物车当前为空
Anti-AK3L1 Antibody (1J714)
还可以产品编号 TMAY-02328
Anti-AK3L1 Antibody (1J714) 是一种 Mouse 抗体，靶向 AK3L1。Anti-AK3L1 Antibody (1J714) 可用于 FCM,ICC/IF。
Anti-AK3L1 Antibody (1J714)
Anti-AK3L1 Antibody (1J714)
还可以产品编号 TMAY-02328
Anti-AK3L1 Antibody (1J714) 是一种 Mouse 抗体，靶向 AK3L1。Anti-AK3L1 Antibody (1J714) 可用于 FCM,ICC/IF。
产品信息正在更新中，如果您想购买，请点击大包装&定制按钮.
大包装 & 定制
联系我们获取更多批次信息
资源下载
产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-AK3L1 Antibody (1J714) is a Mouse antibody targeting AK3L1. Anti-AK3L1 Antibody (1J714) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 1J714 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human AK4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human AK4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human AK4 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human AK4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 (rh AK4 / Adenylate Kinase 4 / AK3L1; TMPY-04469; P27144-1; Ala2-Tyr223). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member theadenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | PE |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Human AK4 / Adenylate Kinase 4 / AK3L1 protein (TMPY-04469) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
评论列表
预览
Related Tags: buy Anti-AK3L1 Antibody (1J714) | purchase Anti-AK3L1 Antibody (1J714) | Anti-AK3L1 Antibody (1J714) cost | order Anti-AK3L1 Antibody (1J714)
嗨！有任何问题？点我咨询
评论内容