Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MMAF (MonoMethyl auristatin F) 是一种抗有丝分裂剂,通过阻断微管蛋白的聚合来抑制细胞分裂,用作抗肿瘤药物和抗体偶联药物的细胞毒性成分。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 283 | 现货 | ||
2 mg | ¥ 395 | 现货 | ||
5 mg | ¥ 638 | 现货 | ||
10 mg | ¥ 973 | 现货 | ||
25 mg | ¥ 1,470 | 现货 | ||
50 mg | ¥ 1,980 | 现货 | ||
100 mg | ¥ 2,770 | 现货 | ||
200 mg | ¥ 3,970 | 现货 |
产品描述 | MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin. |
靶点活性 | H3397:105 nM. |
体外活性 | MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1]. |
体内活性 | The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[1]. |
细胞实验 | Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[2]. |
别名 | 一甲基澳瑞他汀 F, MonoMethyl auristatin F |
分子量 | 731.96 |
分子式 | C39H65N5O8 |
CAS No. | 745017-94-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 20 mg/mL (27.32 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.3662 mL | 6.831 mL | 13.6619 mL | 34.1549 mL |
5 mM | 0.2732 mL | 1.3662 mL | 2.7324 mL | 6.831 mL | |
10 mM | 0.1366 mL | 0.6831 mL | 1.3662 mL | 3.4155 mL | |
20 mM | 0.0683 mL | 0.3415 mL | 0.6831 mL | 1.7077 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
MMAF 745017-94-1 Cytoskeletal Signaling Others Microtubule Associated Monomethylauristatin F inhibit Microtubule/Tubulin ADC Payload ADC Cytotoxin 一甲基澳瑞他汀 F Inhibitor MonoMethyl auristatin F inhibitor