Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ML-SA1 (Mucolipin synthetic agonist 1) 是一种选择性的TRPML 激动剂,通过促进溶酶体的酸化和蛋白酶活性抑制登格病毒 2 和寨卡病毒,抗 DENV2 RNA 和 ZIKV RNA 的IC50分别为 8.3 μM 和 52.99 μM。ML SA1可诱发自噬。ML SA1可用于广谱抗病毒研究。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 195 | 现货 | ||
5 mg | ¥ 443 | 现货 | ||
10 mg | ¥ 697 | 现货 | ||
25 mg | ¥ 1,390 | 现货 | ||
50 mg | ¥ 2,310 | 现货 | ||
100 mg | ¥ 3,490 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 556 | 现货 |
产品描述 | ML-SA1 (Mucolipin synthetic agonist 1) is a selective TRPML agonist, inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 value of ML-SA1 against DENV2 RNA and ZIKV RNA is 8.3 μM and 52.99 μM, respectively[1]. |
靶点活性 | ZIKV:52.99 μM (IC50), DENV-2:8.3 μM (IC50) |
体外活性 |
ML-SA1 (25 μM; 0~14 hours; A549 cells) possibly affects the entry of DENV2 into host cells[1]. ML-SA1 (0~200 μM; A549 cells) shows that there is no cytotoxicity to the cell line observed, even at concentrations up to 200 μM. ML-SA1 (0~50 μM; A549 cells) significantly suppresses DENV2 at the RNA levels and the IC50 is 8.93 μM[1]. ML-SA1 results in a dose-dependent decrease in ZIKV in A549 cells at both the RNA and protein levels, and the IC50 value of ML-SA1 against ZIKV RNA is 52.99 μM. ML-SA1, as an activator of TRPMLs, appears to be a potent inhibitor of DENV2 and ZIKV in vitro. ML-SA1 promotes lysosomal acidification and protease activity to inhibit viral infection. ML-SA1 can induce autophagy in Huh7 cells or A549 cells[1]. |
别名 | Mucolipin synthetic agonist 1 |
分子量 | 362.42 |
分子式 | C22H22N2O3 |
CAS No. | 332382-54-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 17 mg/mL (46.90 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | 68.9807 mL |
5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | 13.7961 mL | |
10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL | 6.8981 mL | |
20 mM | 0.138 mL | 0.6898 mL | 1.3796 mL | 3.449 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ML-SA1 332382-54-4 Membrane transporter/Ion channel Others TRP/TRPV Channel lysosomal ML SA1 Inhibitor inhibit protease Transient receptor potential channels autophagy broad-spectrum selective Mucolipin synthetic agonist 1 acidification Zika MLSA1 ML-SA-1 antiviral TRP Channel virus Dengue inhibitor