orotidine

Orotidine (Uridin-6-carboxylic acid) 是一种存在于细菌、真菌和植物中的核苷。 在人类中，乳清苷以其 5'-磷酸(乳清酸)形式存在。

Orotidine 5′-monophosphate is a pyrimidine ribonucleoside and plays a role as an endogenous metabolite of human, E. coli or mouse. Orotidine 5′-monophosphate is an intermediate in the biosynthesis of uridine monophosphate (UMP). Orotidine 5′-monophosphate can be used for the study of mechanism of orotidine 5′-monophosphate decarboxylase.

Orotidine 5'-monophosphate trisodium 是一种嘧啶核苷酸。Orotidine 5'-monophosphate trisodium 是通过从头合成途径合成的，用于在大量微生物中合成 DNA，包括 M. tuberculosis、S. cerevisiae、S. typhimurium 和 P. falciparum 等等。乳清酸磷酸核糖基转移酶 (OPRT) 以磷酸核糖焦磷酸 (PRPP) 和乳清酸 (OA) 作为底物催化合成 Orotidine 5'-monophosphate trisodium[1]

5-Fluoroorotic acid (monohydrate) (5-Fluorouracil-6-carboxylic acid Mono(hydrate); 5-FOA) 是一种用于分析酵母分子遗传结构中URA3基因的表达。酵母菌若含有活跃的URA3基因（该基因负责编码乳清酸苷-5'-单磷酸脱羧酶），就能将5-FOA转换成对细胞有害的氟脱氧尿苷。当培养基添加尿嘧啶时，具有URA3基因突变的抗性酵母菌株能在5-FOA的环境下生存。

Uridine-5’-monophosphate (UMP) is a ribonucleotide.1It is formedviadecarboxylation of orotidine-5’-monophosphate (5’-OMP) by OMP decarboxylase. UMP is further phosphorylated by UMP-CMP kinase to form UDP and UTP during nucleic acid biosynthesis. Formulations containing UMP have been used as dietary supplements. 1.Berg, J.M., Tymoczko, J.L., and Stryer, L.Nucleotide BiosynthesisBiochemistry(2002)