fluo-3ff

Fluo-3FF is a fluorescent calcium indicator. It is a di-fluorinated analog of Fluo-3 that displays about a 100-fold lower affinity than Fluo-3 for calcium (Kds = 42 and 0.4 μM, respectively). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Fluo-3FF has excitation emission maxima of approximately 506 and 526 nm, respectively.

Fluo-3FF AM 是一种荧光 Ca2+ 敏感指示剂，低亲和力 (Kd = 42 μM)，激发和发射极值分别为 462 nm 和 526 nm。该化合物对 Mg2+ 不敏感，光稳定性较高。作为 Fluo-3FF AM 的类似物，它在未结合钙时基本无荧光，而结合钙后显示出显著的荧光增强效果。