CalceinOrange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe CalceinOrange . CalceinOrange is retained by living cells and displays excitation emission maxima of 525 550 nm, respectively. CalceinOrange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.