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UBE2K Protein, Human, Recombinant (His & SUMO)

产品编号 TMPJ-01039

UBE2K Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis and SUMO tag. The predicted molecular weight is 38 KDa and the accession number is P61086.

UBE2K Protein, Human, Recombinant (His & SUMO)

UBE2K Protein, Human, Recombinant (His & SUMO)

产品编号 TMPJ-01039
UBE2K Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis and SUMO tag. The predicted molecular weight is 38 KDa and the accession number is P61086.
规格价格库存数量
10 μg
¥ 220
5日内发货
50 μg
¥ 700
5日内发货
500 μg
¥ 4,740
5日内发货
1 mg
¥ 7,510
5日内发货
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常见问题解答
蛋白能保证活性吗?
我们有现货的产品,有活性数据的话会展示在官网生物活性那栏,没有数据的也不代表就一定没有活性,是我们还没有建立活性检测方法,没有进行测试,但是我们的蛋白都是优先按照有活性的方法去表达纯化的。
重组蛋白产品是如何运输的?
大部分重组蛋白产品为冻⼲粉,对温度不敏感,在室温下稳定,一般室温或蓝冰运输。对于液体类蛋白,一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化,无需担心,蛋白可以正常使用,短时间内不会影响产品质量。如果特定蛋白在室温下不稳定,我们会提供相应的低温运输方式,以确保产品质量不受影响。
重组蛋白有吗?
您需要什么种属的蛋白呢,对表达系统和标签有要求吗,用于什么实验?
如何将冻干的重组蛋白配置为储备液?
在打开盖⼦之前,先使用 10000-12000 rpm 的转速进行离心,20-30 秒,将附着在管盖或管壁上的冻⼲粉收集到管底,以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂,产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL,分装储存避免反复冻融。
为什么某些重组蛋白溶液中还需要添加载体蛋白?
载体蛋白通常被用于提高蛋白的稳定性,防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力,这可能导致蛋白和管壁之间难以分离,导致溶液中蛋白的实际浓度下降,从而影响其活性。为减少这种损失,在长期保存重组蛋白 产品之前,建议添加常用的载体蛋白溶液,例如0.1% BSA(牛血清白蛋白)、5% HSA(人血清白蛋白)、10% FBS(胎牛血清)或 5% 海藻糖等。如果重组蛋白的浓度较低,强烈建议添加适量的载体蛋白。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
UBE2K Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis and SUMO tag. The predicted molecular weight is 38 KDa and the accession number is P61086.
种属
Human
表达系统
E. coli
标签N-6xHis, SUMO
蛋白编号P61086
别名
Ubiquitin-Protein Ligase,Ubiquitin-Conjugating Enzyme E2-25K,Ubiquitin-Conjugating Enzyme E2-25 kDa,Ubiquitin-Conjugating Enzyme E2(25K),Ubiquitin-Conjugating Enzyme E2 K,Ubiquitin Carrier Protein,UBE2K,Huntingtin-Interacting Protein 2,HIP-2,HIP2
氨基酸序列
Met1-Asn200
蛋白构建
Met1-Asn200
蛋白纯度
Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
分子量38 KDa (reducing condition)
内毒素< 1 EU/μg by the LAL method.
缓冲液Supplied as a 0.2 μm filtered solution of 20 mM PB, 8% Sucrose, 100 mM NaCl, 0.05% Tween 80, pH 7.5.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Ubiquitin-Conjugating Enzyme E2 K (UBE2K) belongs to the E2 Ubiquitin-Conjugating Enzyme family. UBE2K is highly expressed in the brain, with highest levels found in cortex and striatum, and at lower levels in cerebellum and brainstem. UBE2K may mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. UBE2K is associated with the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with huntingtin, and MHC-heavy chain proteins.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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