首页工具

登录

Serpin A1 Protein, Rat, Recombinant (His)

产品编号 TMPY-00442

别名: SerpinA1, serpin peptidase inhibitor, clade A (α-1 antiproteinase, antitrypsin), member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1

Serpin A1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.2 kDa and the accession number is A0A0G2JY31.

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

TargetMol

Serpin A1 Protein, Rat, Recombinant (His)

规格	价格/CNY		货期	数量
50 μg	￥ 4,460		5日内发货

大包装超大折扣，点击咨询

更多批次查询请联系客服

生物活性

技术参数

产品性质

参考文献

生物活性	Testing in progress
产品描述	Serpin A1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.2 kDa and the accession number is A0A0G2JY31.

种属	Rat
表达系统	HEK293 Cells
标签	C-His
蛋白编号	A0A0G2JY31
别名	SerpinA1, serpin peptidase inhibitor, clade A (α-1 antiproteinase, antitrypsin), member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
蛋白构建	The Rat SERPINA1 (NP_071964.2) (Met1-Arg411) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	45.2 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. Its association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP). Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.

参考文献

1. Khnlein T,et al.(2008) Alpha-1 antitrypsin deficiency: pathogenesis, clinical presentation, diagnosis, and treatment. Am J Med. 121(1): 3-9. 2. Camelier AA,et al.(2008) Alpha-1 antitrypsin deficiency: diagnosis and treatment. J Bras Pneumol. 34(7): 514-27. 3. Subramaniyam D,et al.(2010) Cholesterol rich lipid raft microdomains are gateway for acute phase protein, SERPINA1. Int J Biochem Cell Biol. 42(9): 1562-70. 4. Kilty SJ,et al.(2010) Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy. Am J Rhinol Allergy. 24(1): e4-9.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

=

Mass (in vial)

÷

Desired Reconstitution Concentration

bottom

技术支持

您可能有的问题的答案可以在重组蛋白操作手册中找到

Keywords

Serpin A1 Protein, Rat, Recombinant (His) SerpinA1 serpin peptidase inhibitor, clade A (α-1 antiproteinase, antitrypsin), member 1 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 recombinant recombinant-proteins proteins protein

我们的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

Copyright © 2015-2024 TargetMol Chemicals Inc. All rights reserved. 沪ICP备20019793号-4

沪公网安备 31010602006700号

沪公网安备 31010602006700号

TargetMol Loading

陶术

生物

TargetMol^®中国区唯一合作伙伴

点击进入陶术生物官网

陶术生物

联系我们

电话：

400-820-0310

邮箱：

service@tsbiochem.com

QQ：

2881945090（终端用户）

2885109491（经销商）

经销：

终端：

地址：

上海市静安区江场三路238号8楼