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NAD-ME Protein, Human, Recombinant (His)

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货号 TMPH-04029

别名 NAD-ME, NAD-dependent malic enzyme, mitochondrial, mitochondrial, ME2, Malic enzyme 2

NAD-ME Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P23368.

NAD-ME Protein, Human, Recombinant (His)
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NAD-ME Protein, Human, Recombinant (His)

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货号 TMPH-04029 别名 NAD-ME, NAD-dependent malic enzyme, mitochondrial, mitochondrial, ME2, Malic enzyme 2

NAD-ME Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P23368.

规格价格库存数量
5 μg
¥ 938
5日内发货
10 μg
¥ 1,550
6-8日内发货
20 μg
¥ 2,670
6-8日内发货
50 μg
¥ 5,450
6-8日内发货
100 μg
¥ 9,370
5日内发货
200 μg
¥ 13,200
6-8日内发货
500 μg
¥ 20,900
5日内发货
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产品介绍

生物活性
产品描述
NAD-ME Protein, Human, Recombinant (His) is expressed in E. coli with C-6xHis. The accession number is P23368.
生物活性
Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
种属
Human
表达系统
E. coli
标签C-6xHis
蛋白编号P23368
氨基酸序列MLHIKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGLYQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIFPGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQEVSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYEWPESASSPPVITE+HHHHHH
蛋白构建19-584 aa
蛋白纯度
>95% as determined by SDS-PAGE.
缓冲液Lyophilized from a 0.2 μm filtered PBS, pH 7.4.
复溶方法Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名NAD-ME, NAD-dependent malic enzyme, mitochondrial, mitochondrial, ME2, Malic enzyme 2
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量64.4 kDa (Predicted)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

剂量转换

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技术支持

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关键词

ME 2Malic enzyme2
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