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MAGOH Protein, Human, Recombinant (His)

产品编号 TMPY-01557

MAGOH (Mago Homolog, Exon Junction Complex Subunit) is a Protein Coding gene. MAGOH is the human homolog of Drosophila mago nashi, a protein that is required for normal germplasm development in the Drosophila embryo. In mammals, mRNA expression is not limited to the germplasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. The exon junction complex (EJCs) are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Diseases associated with MAGOH include Metaphyseal Chondrodysplasia, Schmid Type, and Primary Autosomal Recessive Microcephaly.

MAGOH Protein, Human, Recombinant (His)

MAGOH Protein, Human, Recombinant (His)

产品编号 TMPY-01557
MAGOH (Mago Homolog, Exon Junction Complex Subunit) is a Protein Coding gene. MAGOH is the human homolog of Drosophila mago nashi, a protein that is required for normal germplasm development in the Drosophila embryo. In mammals, mRNA expression is not limited to the germplasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. The exon junction complex (EJCs) are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Diseases associated with MAGOH include Metaphyseal Chondrodysplasia, Schmid Type, and Primary Autosomal Recessive Microcephaly.
规格价格库存数量
100 μg
¥ 3,820
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
抑制剂能否用于细胞实验?
可以的,我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
收到产品发现冰盒都化了,怎么办?
一般化合物粉末对温度不敏感,加冰盒是为了防止极端气温,如果冰盒融化不影响产品质量。
是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释?
大部分情况下,都是可以溶解的。但有时,有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释,再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。 比如细胞实验中希望终浓度为 1 μM 的话,可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM,再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液,终浓度即为 1 μM。 为避免药物析出,稀释前,可将母液和培养基 37℃ 预热,避免温度低造成严重析出。若稀释过程中出现化合物析出的情况,建议您采用超声加热的方法使其复溶。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
MAGOH (Mago Homolog, Exon Junction Complex Subunit) is a Protein Coding gene. MAGOH is the human homolog of Drosophila mago nashi, a protein that is required for normal germplasm development in the Drosophila embryo. In mammals, mRNA expression is not limited to the germplasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. The exon junction complex (EJCs) are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Diseases associated with MAGOH include Metaphyseal Chondrodysplasia, Schmid Type, and Primary Autosomal Recessive Microcephaly.
种属
Human
表达系统
E. coli
标签C-His
蛋白编号P61326-1
别名
mago-nashi homolog, proliferation-associated (Drosophila),MAGOHA,MAGOH1
蛋白构建
A DNA sequence encoding the human MAGOH (NP_002361.1) (Met1-Ile146) was expressed with a polyhistide tag at the C-terminus. Predicted N terminal: Met
蛋白纯度
> 90 % as determined by SDS-PAGE
分子量18 kDa (predicted); 18 kDa (reducing conditions)
内毒素Please contact us for more information.
缓冲液Supplied as sterile 137 mM NaCl, 2. 7 mM KCl, 10 mM Na2HPO4, 1. 8 mM KH2PO4, 20% glycerol, pH 4. 5.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
MAGOH (Mago Homolog, Exon Junction Complex Subunit) is a Protein Coding gene. MAGOH is the human homolog of Drosophila mago nashi, a protein that is required for normal germplasm development in the Drosophila embryo. In mammals, mRNA expression is not limited to the germplasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. The exon junction complex (EJCs) are deposited on messenger RNAs (mRNAs) during splicing and their core consists of eIF4A3, MLN51, Y14, and MAGOH. Both MAGOH proteins interact with other EJC components, incorporate into mRNA-bound EJCs, and activate nonsense-mediated decay. Diseases associated with MAGOH include Metaphyseal Chondrodysplasia, Schmid Type, and Primary Autosomal Recessive Microcephaly.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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