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Interferon alpha 2/IFNA2 Protein, Human, Recombinant

产品编号 TMPY-00265

Interferon alpha 2/IFNA2 Protein, Human, Recombinant is expressed in yeast. The predicted molecular weight is 19.3 kDa and the accession number is P01563.1.

Interferon alpha 2/IFNA2 Protein, Human, Recombinant

Interferon alpha 2/IFNA2 Protein, Human, Recombinant

产品编号 TMPY-00265
Interferon alpha 2/IFNA2 Protein, Human, Recombinant is expressed in yeast. The predicted molecular weight is 19.3 kDa and the accession number is P01563.1.
规格价格库存数量
100 μg
¥ 3,430
现货
200 μg
¥ 6,070
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
小规格产品,收到瓶子后看不到任何东西?
由于产品规格小,粉末在运输过程中产生的静电导致过于分散,管壁和盖子上皆会粘着,您可以放心,离心后加入适量溶剂直接配制母液、充分溶解即可。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
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产品信息

生物活性
1.Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The ED50 for this effect is typically 0.6-4.2 pg/mL._x000D_ 2.Loaded IFNAR2 Protein, Cynomolgus, Recombinant (His) (Cat#TMPY-00139) on NTA Biosensor, can bind Interferon alpha 2/IFNA2 Protein, Human, Recombinant (Cat#TMPY-00265) with an affinity constant of 23.6 nM as determined in BLI assay (Sartorius Octet RED384).
Interferon alpha 2/IFNA2 Protein, Human, RecombinantInterferon alpha 2/IFNA2 Protein, Human, Recombinant
产品描述
Interferon alpha 2/IFNA2 Protein, Human, Recombinant is expressed in yeast. The predicted molecular weight is 19.3 kDa and the accession number is P01563.1.
种属
Human
表达系统
P. pastoris (Yeast)
标签Tag Free
蛋白编号P01563.1
别名
干扰素,interferon, α2,interferon, alpha 2,Interferon α2/IFNA2,Interferon α2,Interferon alpha 2,INFA2,IFN-αA,IFN-alphaA,IFNAB,IFNA2B,IFNA
蛋白构建
A DNA sequence encoding the human IFNA2 (P01563.1) (Cys24-Glu188) was expressed. Predicted N terminal: Cys 24
蛋白纯度
≥ 90 % as determined by SDS-PAGE.
Interferon alpha 2/IFNA2 Protein, Human, Recombinant
分子量19.3 kDa (predicted)
内毒素Please contact us for more information.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant.

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