购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

HPN Protein, Human, Recombinant (His)

产品编号 TMPK-00778

HPN Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 42 kDa and the accession number is P05981.

HPN Protein, Human, Recombinant (His)

HPN Protein, Human, Recombinant (His)

产品编号 TMPK-00778
HPN Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 42 kDa and the accession number is P05981.
规格价格库存数量
100 μg
¥ 2,960
5日内发货
500 μg
¥ 11,800
5日内发货
1 mg
¥ 19,700
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
重组蛋白产品是如何运输的?
大部分重组蛋白产品为冻⼲粉,对温度不敏感,在室温下稳定,一般室温或蓝冰运输。对于液体类蛋白,一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化,无需担心,蛋白可以正常使用,短时间内不会影响产品质量。如果特定蛋白在室温下不稳定,我们会提供相应的低温运输方式,以确保产品质量不受影响。
重组蛋白产品该如何保存?
1) 存储条件:重组蛋白产品收到后,如不立即使用,应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上,液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完,如果不能在一周内用完,需在 -20℃ 至 -80℃ 保存,可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融,建议将重组蛋白产品分装成多份。分装冻存时,建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL,且每管的体积不小于 20 uL。
蛋白能保证活性吗?
我们有现货的产品,有活性数据的话会展示在官网生物活性那栏,没有数据的也不代表就一定没有活性,是我们还没有建立活性检测方法,没有进行测试,但是我们的蛋白都是优先按照有活性的方法去表达纯化的。
重组蛋白有吗?
您需要什么种属的蛋白呢,对表达系统和标签有要求吗,用于什么实验?
为什么某些重组蛋白溶液中还需要添加载体蛋白?
载体蛋白通常被用于提高蛋白的稳定性,防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力,这可能导致蛋白和管壁之间难以分离,导致溶液中蛋白的实际浓度下降,从而影响其活性。为减少这种损失,在长期保存重组蛋白 产品之前,建议添加常用的载体蛋白溶液,例如0.1% BSA(牛血清白蛋白)、5% HSA(人血清白蛋白)、10% FBS(胎牛血清)或 5% 海藻糖等。如果重组蛋白的浓度较低,强烈建议添加适量的载体蛋白。
查看更多

产品信息

生物活性
Immobilized Human HPN, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 10.4ng/ml determined by ELISA.
产品描述
HPN Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 42 kDa and the accession number is P05981.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号P05981
别名
TMPRSS1,HX,Hpxn,HPX,HPN,Hepsin,Hemopexin,FLJ56652
蛋白构建
Arg45-Leu417
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量42 kDa (predicted). Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Home parenteral nutrition (HPN) has been proposed as the treatment of choice in patients suffering from intestinal failure (IF) and has been claimed to improve survival and quality of life either in patients with benign disorders or even in those with malignancies. HPN seems to have beneficial effect but it should be considered with caution by the physicians who should take into account the indications of each patient to receive parenteral nutrition, the underlying disease and prognosis and the access of each patient to home care services.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

关键词