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HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 365 | 3日内发货 | |
| 10 μg | ¥ 585 | 3日内发货 | |
| 25 μg | ¥ 1,200 | 现货 | |
| 50 μg | ¥ 1,880 | 5日内发货 | |
| 100 μg | ¥ 3,170 | 5日内发货 | |
| 500 μg | ¥ 13,500 | 5日内发货 | |
| 1 mg | ¥ 18,000 | 5日内发货 |
| 生物活性 | Immobilized Human HGF R, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human HGF, hFc Tag with the EC50 of 10.1 ng/ml determined by ELISA. (QC Test) Immobilized Human HGF R, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 15.6 ng/ml determined by ELISA. |
| 产品描述 | HGFR/c-Met Protein, Human, Recombinant (His) V2 is expressed in HEK293 mammalian cells with His tag. The accession number is P08581-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | P08581-1 |
| 别名 | Tyrosine-protein kinase Met,SF receptor,Proto-oncogene c-Met,MET,HGF/SF receptor,HGF receptor,Hepatocyte growth factor receptor |
| 蛋白构建 | Glu25-Thr932 |
| 蛋白纯度 | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| 分子量 | 32.5 kDa (α chain) and 72.1 kDa (β chain) (Predicted); 45-60 kDa (α subunit) and 80-100 kDa (β subunit) (Reducing conditions due to glycosylation) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. |