GLB1 Protein, Human, Recombinant (His)

产品编号 TMPJ-00869

β Galactosidase is a lysosomal β Galactosidase that hydrolyzes the terminal β Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature β Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of β Galactosidase results a catalytically inactive β Galactosidase that plays an important role in vascular development. Defects of β-galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for β Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.

GLB1 Protein, Human, Recombinant (His)

产品编号 TMPJ-00869

规格	价格	库存
10 μg	¥ 910	5日内发货
50 μg	¥ 2,760	5日内发货
500 μg	¥ 12,100	5日内发货
1 mg	¥ 17,400	5日内发货

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常见问题解答

问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问细胞实验中，抑制剂如何溶解？

答用 DMSO 配置较高浓度母液，母液存于 -80℃，建议多分装几管，一周内使用的存于 4℃，避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释，细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%，则需要在做预试验的时候，先进行溶剂阴性对照实验，排除溶剂对细胞的影响。

问动物不能耐受 DMSO，给药时 DMSO量应该如何控制？

答对于普通的老鼠，DMSO 的浓度应控制在 10% 以下，对于裸鼠、转基因小鼠、耐受性弱的老鼠等，DMSO 浓度需控制在2%以下。对于首次操作的抑制剂，建议先做溶剂阴性对照，确认溶解对动物无非特异性影响。

问超声久了会不会对化合物的结构有影响？

答建议以较低频率对化合物进行超声，不会对化合物的结构造成影响。

问收到粉状试剂粘壁严重，如何处理？

答建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	β Galactosidase is a lysosomal β Galactosidase that hydrolyzes the terminal β Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature β Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of β Galactosidase results a catalytically inactive β Galactosidase that plays an important role in vascular development. Defects of β-galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for β Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.
种属	Human
表达系统	HEK293 Cells
标签	C-6xHis
蛋白编号	P16278
别名	β-Galactosidase,Lactase,GLB1,ELNR1,Elastin Receptor 1,Beta-Galactosidase,Acid β-Galactosidase,Acid Beta-Galactosidase
氨基酸序列	Leu24-Val677
蛋白构建	Leu24-Val677
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	90 KDa (reducing condition)
内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	β Galactosidase is a lysosomal β Galactosidase that hydrolyzes the terminal β Galactose from Ganglioside and Keratan sulfate. In lysosome, the mature β Galactosidase protein associates with Cathepsin A and Neuraminidase 1 to form the lysosomal multienzyme complex . An alternative splicing at the RNA level of β Galactosidase results a catalytically inactive β Galactosidase that plays an important role in vascular development. Defects of β-galactosidase (GLB1) are the cause of diseases like GM1-gangliosidosis which is a lysosomal storage disease and Morquio Syndrome B that cause patients to have abnormal elastic fibers. More than 100 mutations have been identified for β Galactosidase, which result in different residual activities of the mutant enzymes and a spectrum of symptoms in the two related diseases.

GLB1 Protein, Human, Recombinant (His)

GLB1 Protein, Human, Recombinant (His)

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