FOLR2 重组蛋白, Cynomolgus (His)

FOLR2 Protein, Cynomolgus, Recombinant (His)

产品编号 TMPK-00485

FOLR2 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25.5 kDa and the accession number is A0A2K5U027-1.

规格	价格	库存
100 μg	¥ 2,660	5日内发货
500 μg	¥ 10,600	5日内发货
1 mg	¥ 17,800	5日内发货

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常见问题解答

问是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释？

答大部分情况下，都是可以溶解的。但有时，有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释，再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。比如细胞实验中希望终浓度为 1 μM 的话，可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM，再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液，终浓度即为 1 μM。为避免药物析出，稀释前，可将母液和培养基 37℃ 预热，避免温度低造成严重析出。若稀释过程中出现化合物析出的情况，建议您采用超声加热的方法使其复溶。

问抑制剂产品应如何储存？

答粉末储存在 -20℃，可以存 3 年以上。母液一般储存于 -80℃，可以存 1 年以上，建议多分装几管，避免反复冻融。常用的存于 4℃，可放一周以上。

问超声一般需要多久？

答超声时长和溶解的量和浓度都有关，一般建议半个小时左右，如果浓度接近最大溶解度，或者需溶解的粉末较多，建议延长超声时间，并辅以加热。

问溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思？

答这句话指的是，如果溶解度 < 1 mg/mL，那我们就认为它是微溶或不溶的，这是针对写的“DMSO：Insoluble”或“DMSO：slightly soluble”这类化合物的。

问Tween-20 可以用 Tween-80 替代吗？

答不建议使用 Tween-20。相较于 Tween-20，Tween-80 的耐受性更好，Tween-80已被用作评估实验药物和毒物的行为影响的工具，且没有明显的副作用。参考文献：Castro CA， Hogan JB， Benson KA， Shehata CW， Landauer MR. Behavioral effects of vehicles: DMSO， ethanol， Tween-20， Tween-80， and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.

产品信息

生物活性	Immobilized Cynomolgus FOLR2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
产品描述	FOLR2 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25.5 kDa and the accession number is A0A2K5U027-1.
种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His
蛋白编号	A0A2K5U027-1
别名	β-HFR,β HFR,OLR2,FR-β,FR-P3,FR-BETA,FR β,FR P3,FR BETA,FOLR2,FBP,BETA-HFR,BETA HFR
蛋白构建	Gln9-His187
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	25.5 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM Glycine (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes; its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy).

FOLR2 Protein, Cynomolgus, Recombinant (His)

FOLR2 Protein, Cynomolgus, Recombinant (His)

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