Erythropoietin 重组蛋白, Horse (hFc)

Erythropoietin Protein, Horse, Recombinant (hFc)

产品编号 TMPH-00827

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin Protein, Horse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFC tag. The predicted molecular weight is 47.3 kDa and the accession number is Q867B1.

Erythropoietin Protein, Horse, Recombinant (hFc)

产品编号 TMPH-00827

规格	价格	库存
20 μg	¥ 3,910	20日内发货
100 μg	¥ 11,900	20日内发货
1 mg	¥ 49,500	20日内发货

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常见问题解答

问抑制剂母液的储存方式？

答母液配置完成后，一般储存于 -80℃，可以存 1 年以上。建议多分装几管，避免反复冻融，常用的存于 4 度，可放一周以上。

问超声久了会不会对化合物的结构有影响？

答建议以较低频率对化合物进行超声，不会对化合物的结构造成影响。

问动物不能耐受 DMSO，给药时 DMSO量应该如何控制？

答对于普通的老鼠，DMSO 的浓度应控制在 10% 以下，对于裸鼠、转基因小鼠、耐受性弱的老鼠等，DMSO 浓度需控制在2%以下。对于首次操作的抑制剂，建议先做溶剂阴性对照，确认溶解对动物无非特异性影响。

问得到的杀死细胞的 IC50 与网页上 IC50 差距较大怎么办？

答您所测试的IC50是指细胞的增殖抑制实验（半抑制率），细胞增殖抑制实验 IC50值，和网页上的靶点IC50其实是不同的意义。文献里和网页上 IC50其实往往是指对于靶点无细胞实验的抑制率，这样的实验一般是通过激酶或者蛋白纯化实验做出来的，nM 级别的浓度比较常见，但是细胞的增殖抑制实验 IC50 是指细胞的半致死率，同时牵涉到细胞的代谢以及渗透，一般浓度会高一些。另外，同一个化合物对不同细胞模型的效果也是不同的，建议尝试增加孵育量、延长孵育时间。

问抑制剂能否用于细胞实验？

答可以的，我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献，这种情况下，建议您先做预实验，确保研究的可行性。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin Protein, Horse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFC tag. The predicted molecular weight is 47.3 kDa and the accession number is Q867B1.
种属	Horse
表达系统	HEK293 Cells
标签	C-hFC
蛋白编号	Q867B1
别名	Erythropoietin,EPO
氨基酸序列	APPRLICDSRVLERYILEAREAENVTMGCAEGCSFGENVTVPDTKVNFYSWKRMEVEQQAVEVWQGLALLSEAILQGQALLANSSQPSETLRLHVDKAVSSLRSLTSLLRALGAQKEAISPPDAASAAPLRTFAVDTLCKLFRIYSNFLRGKLKLYTGEACRRGDR
蛋白构建	27-192 aa
蛋白纯度	> 90% as determined by SDS-PAGE.
分子量	47.3 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Erythropoietin Protein, Horse, Recombinant (hFc)

Erythropoietin Protein, Horse, Recombinant (hFc)

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