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EphB1 Protein, Rhesus, Recombinant (hFc)

产品编号 TMPY-03826

EphB1 Protein, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 85.4 kDa and the accession number is A0A1D5QF63.

EphB1 Protein, Rhesus, Recombinant (hFc)

EphB1 Protein, Rhesus, Recombinant (hFc)

产品编号 TMPY-03826
EphB1 Protein, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 85.4 kDa and the accession number is A0A1D5QF63.
规格价格库存数量
200 μg
¥ 5,750
5日内发货
大包装 & 定制
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产品信息

生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human Ephrin-B1 His at 2 μg/ml (100 μl/well) can bind Rhesus EphB1 hFc, the EC50 of Rhesus EphB1 hFc is 4.0-20.0 ng/mL.
产品描述
EphB1 Protein, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 85.4 kDa and the accession number is A0A1D5QF63.
种属
Rhesus
表达系统
HEK293 Cells
标签C-hFc
蛋白编号A0A1D5QF63
别名
EPH receptor B1
蛋白构建
A DNA sequence encoding the rhesus EPHB1 (XP_001115263.1) (Met1-Pro540) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Met 18
蛋白纯度
> 85 % as determined by SDS-PAGE
分子量85.4 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

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