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CXCL10 Protein, Mouse, Recombinant

产品编号 TMPH-03768

CXCL10 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 8.7 kDa and the accession number is P17515.

CXCL10 Protein, Mouse, Recombinant

CXCL10 Protein, Mouse, Recombinant

产品编号 TMPH-03768
CXCL10 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 8.7 kDa and the accession number is P17515.
规格价格库存数量
5 μg
¥ 1,070
现货
100 μg
¥ 7,600
20日内发货
500 μg
¥ 17,000
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
小规格产品,收到瓶子后看不到任何东西?
由于产品规格小,粉末在运输过程中产生的静电导致过于分散,管壁和盖子上皆会粘着,您可以放心,离心后加入适量溶剂直接配制母液、充分溶解即可。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
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产品信息

生物活性
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1- 10.0 ng/mL in the presence of IL-2.
产品描述
CXCL10 Protein, Mouse, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 8.7 kDa and the accession number is P17515.
种属
Mouse
表达系统
E. coli
标签Tag Free
蛋白编号P17515
别名
Small-inducible cytokine B10,Scyb10,Interferon-gamma induced protein CRG-2,Inp10,Ifi10,Cxcl10,C-X-C motif chemokine 10,Crg2,C7,10 kDa interferon gamma-induced protein (Gamma-IP10;IP-10)
氨基酸序列
IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP
蛋白构建
22-98 aa
蛋白纯度
>97% as determined by SDS-PAGE.
分子量8.7 kDa as predicted
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液0.2 μm filtered 2×PBS, pH 7.4, lyophilized.
复溶方法
A hardcopy of COA with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
(C-X-C motif) ligand (CXCL)10 (CXCL10) belongs to the ELR(-) CXC subfamily chemokine. CXCL10/IP-10 exerts its function through binding to chemokine (C-X-C motif) receptor 3 (CXCR3), a seven trans-membrane receptor coupled to G proteins. CXCL10/IP-10 and its receptor, CXCR3, appear to contribute to the pathogenesis of many autoimmune diseases, organ specific (such as type 1 diabetes, autoimmune thyroiditis, Graves' disease and ophthalmopathy), or systemic (such as rheumatoid arthritis, psoriatic arthritis, systemic lupus erythematosus, mixed cryoglobulinemia, Sjögren syndrome, or systemic sclerosis). CXCL10/IP-10 is secreted by several cell types including endothelial cells, fibroblasts, keratinocytes, thyrocytes, preadipocytes, etc. Determination of high level of CXCL10/IP-10 in peripheral fluids is therefore a marker of host immune response.

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