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B7-H3 Protein, Human, Recombinant (His), PE-Labeled

产品编号 TMPY-07061

B7-H3 Protein, Human, Recombinant (His), PE-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.

B7-H3 Protein, Human, Recombinant (His), PE-Labeled

B7-H3 Protein, Human, Recombinant (His), PE-Labeled

产品编号 TMPY-07061
B7-H3 Protein, Human, Recombinant (His), PE-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.
规格价格库存数量
25 tests
¥ 2,930
期货
100 tests
¥ 9,870
期货
大包装 & 定制
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TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
超声应使用什么样的频率?
频率建议使用常规的超声清洗频率即可,一般在20 kHz - 100 kHz。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
产品溶解需要超声吗?如果没有超声仪怎么办?
超声可以加快粉末的溶解速度,如果您的化合物溶解不了,建议您尝试超声。如果您没有超声仪,建议您选择较低母液浓度,或者较少粉末量,尝试溶解。
动物不能耐受 DMSO,给药时 DMSO量应该如何控制?
对于普通的老鼠,DMSO 的浓度应控制在 10% 以下,对于裸鼠、转基因小鼠、耐受性弱的老鼠等,DMSO 浓度需控制在2%以下。对于首次操作的抑制剂,建议先做溶剂阴性对照,确认溶解对动物无非特异性影响。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
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产品信息

生物活性
1. 5e5 of 293-CAR cells expressing the anti-B7-H3 scFv can be directly stained with 100 μL (2 μL stock solution diluted to 100 μL FCM buffer) of B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and the percentage of CAR-expressing cells can be detected by flow cytometry. Non-transduced 293 cells was used as a control (QC tested).
2. 5e5 of PBMCs can be stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and detected by flow cytometry (QC tested).
3. Flow cytometric analysis of anti-B7-H3 CAR expression. HEK293 cells were lentivirally transduced with anti-B7-H3 CAR. Flow cytometric analysis was performed with a negative control protein and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061), respectively. Non-transduced 293 cells were used as a control (left).
4. Binding activity of PE-conjugated B7-H3 protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and B7-H3 Protein, Human, Recombinant (His), PE-Labeled (Cat#TMPY-07061) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
产品描述
B7-H3 Protein, Human, Recombinant (His), PE-Labeled is expressed in HEK293 Cells. The accession number is Q5ZPR3-1.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号Q5ZPR3-1
别名
UNQ309/PRO352,PSEC0249,B7RP-2,B7-H3,B7H3,4Ig-B7-H3
蛋白构建
A DNA sequence encoding the human B7-H3 (Q5ZPR3-1) (Met1-Thr461) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with PE (Excitation= 495 - 566 nm, Emission Max.= 575 nm).
分子量50 kDa (predicted)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液This product is Lyophilized from sterile PBS, 0.2% BSA, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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