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B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated

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货号 TMPK-00315

别名 UNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 49.5 kDa and the accession number is Q5ZPR3-1.

B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated

B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated

一键复制产品信息
Rating icon 还可以
SPR兼容缓冲液
货号 TMPK-00315 别名 UNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 49.5 kDa and the accession number is Q5ZPR3-1.

规格价格库存数量
5 μg
¥ 568
6-8日内发货
10 μg
¥ 928
6-8日内发货
20 μg
¥ 1,530
5日内发货
50 μg
¥ 2,970
5日内发货
100 μg
¥ 5,180
5日内发货
200 μg
¥ 9,390
5日内发货
500 μg
¥ 20,700
5日内发货
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产品介绍


生物活性
产品描述
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 (4Ig) /B7-H3b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 49.5 kDa and the accession number is Q5ZPR3-1.
生物活性
Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 7.7ng/ml determined by ELISA.
研究背景
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号Q5ZPR3-1
蛋白构建Gly27-Thr461
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名UNQ309, PSEC0249, PRO352, CD276, B7-H3, B7 homolog 3,  4Ig-B7-H3
内毒素< 1 EU/μg by the LAL method.
化学信息
分子量49.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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