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B7-2/CD86 Protein, Human, Recombinant (His & Avi), Biotinylated
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货号 TMPK-00881
别名 MGC34413, LAB72, FUN-1, CD86 molecule, CD86, CD28LG2, BU63, B7-2 antigen, B7-2, B72, B70
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P42081-1.
其他形式的 “B7-2/CD86 Protein, Human, Recombinant (His & Avi), Biotinylated”:
B7-2/CD86 Protein, Human, Recombinant (His & Avi), Biotinylated
一键复制产品信息 很棒SPR兼容缓冲液
货号 TMPK-00881 别名 MGC34413, LAB72, FUN-1, CD86 molecule, CD86, CD28LG2, BU63, B7-2 antigen, B7-2, B72, B70
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P42081-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 568 | 现货 | |
| 10 μg | ¥ 928 | 现货 | |
| 20 μg | ¥ 1,530 | 现货 | |
| 50 μg | ¥ 2,970 | 3日内发货 | |
| 100 μg | ¥ 5,180 | 现货 | |
| 200 μg | ¥ 9,390 | 5日内发货 | |
| 500 μg | ¥ 20,700 | 5日内发货 |
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产品介绍
生物活性
化学信息
储存&溶解度
| 产品描述 | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P42081-1. |
| 生物活性 | Immobilized Human CTLA-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-2, His Tag with the EC50 of 3.8μg/ml determined by ELISA. |
| 研究背景 | B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His-Avi |
| 蛋白编号 | P42081-1 |
| 蛋白构建 | Leu26-Pro247 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 别名 | MGC34413, LAB72, FUN-1, CD86 molecule, CD86, CD28LG2, BU63, B7-2 antigen, B7-2, B72, B70 |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 分子量 | 28.2 kDa (predicted). Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
| 颜色 | White |
| 物理性状 | Lyophilized powder |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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