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Angiopoietin-2 Protein, Human (His & Avi), Biotinylated
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还可以货号 TMPK-00304
别名 Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
其他形式的 “Angiopoietin-2 Protein, Human (His & Avi), Biotinylated”:
Angiopoietin-2 Protein, Human (His & Avi), Biotinylated
一键复制产品信息 还可以货号 TMPK-00304 别名 Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 5,180 | 5日内发货 | |
| 500 μg | ¥ 20,700 | 5日内发货 |
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| 产品描述 | Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
| 生物活性 | 1. Immobilized Anti-ANGPT2 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ANGPT2, His Tag with the EC50 of 0.12μg/ml determined by ELISA.
2. Human TIE2, His Tag immobilized on CM5 Chip can bind Biotinylated Human ANGPT2, His Tag with an affinity constant of 2.93 μM as determined in SPR assay. |
| 研究背景 | Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Mature Ang-2 is 478 amino acids in length.Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. |
| 种属 | Human |
| 表达系统 | HEK293 |
| 标签 | N-His-Avi |
| 蛋白编号 | O15123-1 |
| 蛋白构建 | Recombinant Biotinylated Human ANGPT2/Angiopoietin-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Lys275-Phe496.[Accession |O15123-1] |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| 别名 | Tie2-ligand, ANGPT2, angiopoitin 2, angiopoietin-2B, angiopoietin-2a, Angiopoietin-2, angiopoietin 2, ANG-2, ANG2, AGPT2 |
| 内毒素 | Less than 1EU per μg by the LAL method. |
| 分子量 | 28.4 kDa (predicted). Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result. |
| 存储 | -20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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