| 生物活性 | Immobilized Human 4-1BB, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human 4-1BB Ligand, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. |
| 产品描述 | 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. 4-1BB/CD137/TNFRSF9 Protein, Human, Recombinant (hFc & Avi) is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 45.7 kDa and the accession number is Q07011. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-hFc-Avi |
| 蛋白编号 | Q07011 |
| 别名 | TNFRSF9,T cell antigen ILA,ILA,FLJ43501,CDw137,CD137,4-1BB ligand receptor,4-1BB,41BB |
| 蛋白构建 | Leu24-Gln186 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 分子量 | 45.7 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
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