Ru(bpy)2(phen-ITC)(PF6)2 is a versatile dye employed extensively across various domains, including biological experimentation and industrial applications. In biological settings, this dye enables scientists to observe and analyze cell structures, track biomolecules, evaluate cellular functions, differentiate between cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its utility extends beyond scientific research, encompassing diagnostic uses in clinical settings. Additionally, Ru(bpy)2(phen-ITC)(PF6)2 finds application in traditional industries like textile dyeing and emerging sectors such as functional textile processing, food coloring, and dye-sensitized solar cells. This broad applicability underscores its significance in both scientific and industrial fields.