Photoacoustic contrast agent-2 (acetate) is a versatile dye that serves as an essential tool in biological experiments. It aids researchers in observing and analyzing cell structures, tracking biomolecules, assessing cellular functions, differentiating cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications range from fundamental scientific research to clinical diagnostics, as well as traditional fields like textile dyeing and emerging areas such as functional textile treatment, food coloring, and dye-sensitized solar cells.