Mucicarmine effectively stains mucin on frozen-tissue sections, appearing deep rose. To perform the staining, mount cryostat sections (4 to 5 μm thick) onto coated glass slides. Fix these sections in frozen-section fixative for 30 seconds, then rinse with water for 5 seconds. Immerse the slides in Harris hematoxylin for 30 seconds, followed by 5-second immersions in distilled water, ammonium hydroxide, and distilled water. Heat the mucicarmine stain in a microwave until it is about to bubble (approximately 30 seconds). Submerge the slides in the warmed mucicarmine solution for 2 minutes and rinse with distilled water for 5 seconds. Sequentially dip the slides in 95% alcohol, 100% alcohol, and xylene for 5 seconds each before coverslipping. Note: Nuclei (blue) and connective tissue (green) staining may be necessary based on specific requirements.

The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands.