CHRG01, a biologically active peptide, originates from the C-terminal amino acids 54 to 67 of human β-defensin 3 (hBD-3), with a modified structure wherein all cysteine (Cys) residues are replaced by serine (Ser) to eliminate disulfide bond linkages. Like its parent compound hBD-3, CHRG01 exhibits antimicrobial properties that are dependent on electrostatic interactions.