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Beta-Tubulin Loading Control Antibody (1L64) 是一种 Rabbit 抗体,靶向 Beta-Tubulin Loading Control。Beta-Tubulin Loading Control Antibody (1L64) 可用于 WB,IHC-P,IHC-Fr,IF。
Beta-Tubulin Loading Control Antibody (1L64) 是一种 Rabbit 抗体,靶向 Beta-Tubulin Loading Control。Beta-Tubulin Loading Control Antibody (1L64) 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 μL | ¥ 785 | 5日内发货 | |
50 μL | ¥ 1,385 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Beta-Tubulin Loading Control Antibody (1L64) is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Antibody (1L64) can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
克隆号 | 1L64 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Human U251 cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human 293T cell lysates Primary: Anti-beta Tubulin (TMAB-00234) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
应用 | |
推荐剂量 | WB: 1:2000-5000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cytoplasmic, cytoskeleton. |
构建方式 | Recombinant Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014] |
免疫原 | A synthesized peptide: human beta Tubulin |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Microtubules,Tubulin |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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