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Beta-Tubulin Loading Control Antibody (1L64)

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Beta-Tubulin Loading Control Antibody (1L64)
产品编号 TMAB-00234

Beta-Tubulin Loading Control Antibody (1L64) 是一种 Rabbit 抗体,靶向 Beta-Tubulin Loading Control。Beta-Tubulin Loading Control Antibody (1L64) 可用于 WB,IHC-P,IHC-Fr,IF。

Beta-Tubulin Loading Control Antibody (1L64)

Beta-Tubulin Loading Control Antibody (1L64)

Rating icon 还可以
Beta-Tubulin Loading Control Antibody (1L64)
产品编号 TMAB-00234

Beta-Tubulin Loading Control Antibody (1L64) 是一种 Rabbit 抗体,靶向 Beta-Tubulin Loading Control。Beta-Tubulin Loading Control Antibody (1L64) 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
25 μL
¥ 785
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50 μL
¥ 1,385
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Beta-Tubulin Loading Control Antibody (1L64) is a Rabbit antibody targeting Beta-Tubulin Loading Control. Beta-Tubulin Loading Control Antibody (1L64) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
克隆号
1L64
交叉反应
Human,Mouse,Rat
验证活性
1. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Human U251 cell lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human 293T cell lysates
Primary: Anti-beta Tubulin (TMAB-00234) at 1/20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta Tubulin Monoclonal Antibody, Unconjugated (TMAB-00234) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
应用
推荐剂量
WB: 1:2000-5000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cytoplasmic, cytoskeleton.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
抗原信息
免疫原
A synthesized peptide: human beta Tubulin
抗原种属
Human
基因ID
Uniprot ID
研究领域
Microtubules,Tubulin
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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