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Anti-VCP Antibody (3I403) 是一种 Rabbit 抗体,靶向 VCP。Anti-VCP Antibody (3I403) 可用于 WB,ICC/IF,IHC,FCM,IP。
Anti-VCP Antibody (3I403) 是一种 Rabbit 抗体,靶向 VCP。Anti-VCP Antibody (3I403) 可用于 WB,ICC/IF,IHC,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-VCP Antibody (3I403) is a Rabbit antibody targeting VCP. Anti-VCP Antibody (3I403) can be used in WB,ICC/IF,IHC,FCM,IP. |
别名 | VCP, Valosin-Containing Protein, Transitional Endoplasmic Reticulum ATPase, TER ATPase, 15S Mg(2+)-ATPase p97 Subunit |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat,zebrafish |
验证活性 | 1. Western blot analysis of VCP on different cells lysates using anti-VCP antibody at 1/500 dilution. Positive control: Line 1: SH-SY5Y, Line 2: HepG2, Line 3: Hela. 2. Western blot analysis of VCP on hybrid fish (crucian-carp) heart tissue lysate using anti-VCP antibody at 1/500 dilution. 3. Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-VCP antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-VCP antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-VCP antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VCP antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VCP antibody. Counter stained with hematoxylin. 8. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VCP antibody. Counter stained with hematoxylin. 9. ICC staining VCP in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining VCP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. ICC staining VCP in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 12. Flow cytometric analysis of HL-60 cells with VCP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Valosin containing protein (VCP), also designated TERA (for transitional endoplasmic reticulum ATPase) or p97, is a member of the AAA family of ATPases, which are involved in a variety of cellular activities. VCP is the mammalian homolog of Saccharomyces cerevisiae Cdc48, a protein essential for the completion of mitiosis in yeast. VCP is thought to be involved in a variety of membrane functions and in the regulation of the cell cycle. It associates with ubiquitinated IκB-α as well as with the 26S Proteosome, indicating a potential role for VCP in the proteosome-mediated degradation of IκB-α. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 89 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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