Anti-TUBB3 Antibody (3P633) is a Rabbit antibody targeting TUBB3. Anti-TUBB3 Antibody (3P633) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

3P633

Human,Mouse,Rat

1. 25 μg total protein per Lane of various lysates probed with Tubulin beta-III monoclonal antibody, unconjugated (TMAB-01920) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated (TMAB-01920) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

WB: 1:1000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1:50-200

Monoclonal

A synthesized peptide: human beta III Tubulin

Human

TUBB3

Tubulin beta-3 chain

Neuronal,Tubulin,Microtubules

Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance.