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Anti-TNNI2 Antibody (1Z708) 是一种 Mouse 抗体,靶向 TNNI2。Anti-TNNI2 Antibody (1Z708) 可用于 WB,IHC,FCM。
Anti-TNNI2 Antibody (1Z708) 是一种 Mouse 抗体,靶向 TNNI2。Anti-TNNI2 Antibody (1Z708) 可用于 WB,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,980 | 5日内发货 | |
100 μL | ¥ 2,995 | 5日内发货 |
产品描述 | Anti-TNNI2 Antibody (1Z708) is a Mouse antibody targeting TNNI2. Anti-TNNI2 Antibody (1Z708) can be used in WB,IHC,FCM. |
别名 | Troponin I, fast-twitch isoform, Troponin I, fast skeletal muscle, Tnni2, fast skeletal muscle |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of TNNI2 on human TNNI2 recombinant protein using anti-TNNI2 antibody at 1/1,000 dilution. 2. Western blot analysis of TNNI2 on HEK293 (1) and TNNI2-hIgGFc transfected HEK293 (2) cell lysate using anti-TNNI2 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human striated muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin. 5. Flow cytometric analysis of NIH/3T3 cells with TNNI2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:100-200; FCM: 1:100-200 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
研究背景 | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between actin and myosin by binding to calcium. Troponin is made up of at least two subunits, which are skeletal muscle skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator), and Troponin T (the link to the the regulator), and Troponin T C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has 2 isoforms: fast and slow. Fast troponin C has two calci um binding sites while slow / cardiac troponin C has a single calcium binding site. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 21 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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