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Anti-TLR4 Polyclonal Antibody 4
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产品编号 TMAB-01857
Anti-TLR4 Polyclonal Antibody 4 是一种 Rabbit 抗体，靶向 TLR4。Anti-TLR4 Polyclonal Antibody 4 可用于 WB,FCM。
Anti-TLR4 Polyclonal Antibody 4
Anti-TLR4 Polyclonal Antibody 4
还可以产品编号 TMAB-01857
Anti-TLR4 Polyclonal Antibody 4 是一种 Rabbit 抗体，靶向 TLR4。Anti-TLR4 Polyclonal Antibody 4 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-TLR4 Polyclonal Antibody 4 is a Rabbit antibody targeting TLR4. Anti-TLR4 Polyclonal Antibody 4 can be used in WB,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human (predicted:Mouse,Rat,Pig,Cow,Sheep) |
| 验证活性 | 1. Sample: Lane 1: H9C2 (Human) Cell Lysate at 30 μg Lane 2: K562 (Human) Cell Lysate at 30 μg Primary: Anti-TLR4 (TMAB-01857) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kDa Observed band size: 110 kDa 2. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TLR4 antibody (TMAB-01857) Dilution: 0.5 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The Toll-like receptor 4 by activating natural immunity, specific immune response involved in the start-up, Toll-like receptor 4 as an important signal transduction transmembrane receptor involved in the toxin-induced inflammation in the pathological process, its mechanisms of control On a growing concern. all regions were either double-stranded or sequenced with an alternatechemistry or covered by high quality data(i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by at least one subclone; and the assembly was confirmed by restriction digest, except on the rare occasion of the clone being a YAC. The following abbreviations are used to associate primary accession numbers given in the feature table with their source databases: Em:, EMBL; Sw:, SWISSPROT; Tr:, TREMBL; Wp:, WORMPEP; Information on the WORMPEP database can be found at. TLR-4 plays an important role in microvascular leakage and leukocyte adhesion under the inflammatory condition associated with nonseptic thermal injury. |
| 免疫原 | KLH conjugated synthetic peptide: human TLR4 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Inflammatory mediators,Regulatory T Cells,Receptors,Macrophage / Inflamm.,TLR Signaling,Host Virus Interaction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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