Anti-TIE2 Polyclonal Antibody is a Rabbit antibody targeting TIE2. Anti-TIE2 Polyclonal Antibody can be used in ICC/IF,FCM.

IgG

Human,Rat (predicted:Mouse,Pig,Cow,Horse)

1. Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (TIE2) polyclonal Antibody, Unconjugated (TMAB-01839) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Blank control (Black line): HUVEC (Black). Primary Antibody (green line): Rabbit Anti-TIE2 antibody (TMAB-01839) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

ICC/IF: 1:100; FCM: 3ug/Test

Polyclonal

KLH conjugated synthetic peptide: human Tie2

Human

Endothelial Markers,Receptor Tyrosine Kinases,Angiogenesis and vasculogenesis,Endothelium,Endothelial Cells,Endothelial Cell Markers,Growth factor receptors