Anti-TFAM Polyclonal Antibody is a Rabbit antibody targeting TFAM. Anti-TFAM Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Rat,Mouse)

Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-MtTF1/TFAM antibody (TMAB-01822)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human MtTF1

Human

Transcription factors/regulators,Transcription Factors,Mitochondrial transcription,Mitochondrial Biogenesis,Mitochondrial markers,Mitochondrial,Mitochondria