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Anti-TCTP/TPT1 Antibody (7X932) 是一种 Rabbit 抗体,靶向 TCTP/TPT1。Anti-TCTP/TPT1 Antibody (7X932) 可用于 WB,ICC/IF,IHC,FCM,IP。
Anti-TCTP/TPT1 Antibody (7X932) 是一种 Rabbit 抗体,靶向 TCTP/TPT1。Anti-TCTP/TPT1 Antibody (7X932) 可用于 WB,ICC/IF,IHC,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-TCTP/TPT1 Antibody (7X932) is a Rabbit antibody targeting TCTP/TPT1. Anti-TCTP/TPT1 Antibody (7X932) can be used in WB,ICC/IF,IHC,FCM,IP. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of TCTP on different lysates using anti-TCTP antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse placenta tissue, Lane 2: Mouse testis tissue, Lane 3: Hela, Lane 4: A431, Lane 5: MCF-7. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TCTP antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TCTP antibody. Counter stained with hematoxylin. 4. ICC staining TCTP in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining TCTP in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining TCTP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of THP-1 cells with TCTP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down syndrome. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 20 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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