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顶部生物活性储存和溶解信息计算器

Anti-STUB1 Antibody (5M430)

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产品编号 TMAC-03909

别名 STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7

Anti-STUB1 Antibody (5M430) 是一种 Rabbit 抗体，靶向 STUB1。Anti-STUB1 Antibody (5M430) 可用于 FCM,ICC,IF,IHC,IP,WB。

Anti-STUB1 Antibody (5M430)

Anti-STUB1 Antibody (5M430)

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Rating icon 还可以
产品编号 TMAC-03909 别名 STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7

Anti-STUB1 Antibody (5M430) 是一种 Rabbit 抗体，靶向 STUB1。Anti-STUB1 Antibody (5M430) 可用于 FCM,ICC,IF,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-STUB1 Antibody (5M430) is a Rabbit antibody targeting STUB1. Anti-STUB1 Antibody (5M430) can be used in FCM,ICC,IF,IHC,IP,WB.
别名STUB1, STIP1 Homology and U Box-Containing Protein 1, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, CHIP, Carboxy Terminus of Hsp70-Interacting Protein, Antigen NY-CO-7
Ig Type
IgG
克隆号
5M430
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution. Positive control: Lane 1: 293, Lane 2: MCF-7, Lane 3: SK-Br-3, Lane 4: Mouse kidney.
2. Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.
6. ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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应用FCMICCIFIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200; IP: 1:10-50; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景CHIP (carboxy terminus of HSP 70-interacting protein), also designated STIP1 homology and U-box containing protein 1, HSPABP2, NY-CO-7, SDCCAG7 and STUB1, is a cytoplasmic E3 ubiquitin ligase that influences protein ubiquitylation. CHIP interacts with Smad1/Smad4 and blocks BMP signaling through the ubiquitin-mediated degradation of Smad proteins. CHIP controls both association of Hsp70/Hsp90 chaperones with ErbB2 and down-regulation of ErbB2 induced by inhibitors of Hsp90. A 1.3-kb transcript is most abundant in striated muscle (heart and skeletal muscle), with lower expression in pancreas and brain.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human STUB1 aa 1-200
抗原种属
human
Uniprot ID
Q9UNE7
化学信息
分子量Theoretical: 35 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

STUB-1STUB 1

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