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Anti-Stathmin 1 Antibody (2X10) 是一种 Rabbit 抗体,靶向 Stathmin 1。Anti-Stathmin 1 Antibody (2X10) 可用于 WB,ICC,IHC,IP,FCM。
Anti-Stathmin 1 Antibody (2X10) 是一种 Rabbit 抗体,靶向 Stathmin 1。Anti-Stathmin 1 Antibody (2X10) 可用于 WB,ICC,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Stathmin 1 Antibody (2X10) is a Rabbit antibody targeting Stathmin 1. Anti-Stathmin 1 Antibody (2X10) can be used in WB,ICC,IHC,IP,FCM. |
别名 | stathmin 1, SMN, PR22, PP19, PP17, OP18, LAP18, Lag, C1orf215 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin. 6. ICC staining Stathmin 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Stathmin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Stathmin 1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with Stathmin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Op18 (for oncoprotein 18, also designated stathmin, prosolin or metablastin) is a conserved, tubulin-associated, intracellular phosphoprotein . Many different phosphorylated forms of Op18 are observed, and it is expressed as two different isoforms. Op18 is considered a critical regulator of microtubulin dynamics and is downregulated by p53. It serves as a transducing protein, via phophorylation, for a variety of cell signaling pathways and involved in both mitosis and differentiation. Op18 is present in many cancers, including breast carcinomas, and is highly expressed in acute leukemias of different subtypes. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 18 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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