Anti-SQSTM1/p62 Antibody (9J396) is a Rabbit antibody targeting SQSTM1/p62. Anti-SQSTM1/p62 Antibody (9J396) can be used in FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

9J396

Human,Rat,Mouse

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (SQSTM1/p62) monoclonal Antibody, Unconjugated (TMAB-01771) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. 25 μg total protein per Lane of various lysates probed with p62 SQSTM1 monoclonal antibody, unconjugated (TMAB-01771) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
3. The HepG2 (H) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-p62 SQSTM1 antibody (TMAB-01771): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS.
4. Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SQSTM1/p62 Monoclonal Antibody, Unconjugated (TMAB-01771) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human Sequestosome 1

Human

SQSTM1

Sequestosome-1

Autophagosome,Autophagosome,Pol II Transcription,Autophagosome,Regulation,NFkB Pathway

Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.